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目的:了解远隔肢体缺血预处理(RLIP)训练对心肺复苏(CPR)后大鼠的行为学和认知功能的影响,以探讨RLIP训练对CRP后大鼠神经功能的保护作用。方法:成年雄性SD大鼠随机分为四组:Sham组、control组、RLIP组和RLIP训练组。Sham组不建立CPR模型及RLIP模型,control组采用室颤(VF)法建立CPR模型,共进行8 min无处理VF,后开始CPR直至大鼠自主循环恢复(ROSC)。RLIP组:建立CPR模型前24-48 h内行远隔肢体缺血预处理。RLIP训练组:对大鼠进行连续2周的远隔肢体缺血预处理训练,建立CPR模型。记录大鼠复苏后1 d、3 d记录大鼠神经功能缺损评分(NDS);复苏后大鼠ST台实验EN1、ST、EN2评分。结果:(1)control组、RLIP组和RLIP训练组的NDS均明显低于Sham组(P<0.05),RLIP组和RLIP训练组CPR后1 d和3 d的NDS均明显优于control组(P<0.05),RLIP组和RLIP训练组无明显差异(P>0.05)。(2)与Sham组相比,control组、RLIP组和RLIP训练组大鼠EN1和EN2值均增高、ST时间均缩短(P<0.05),RLIP组和RLIP训练组学习和记忆能力明显优于control组(P<0.05),RLIP组和RLIP训练组无明显差异(P>0.05)。结论:RLIP训练可减轻CPR后大鼠神经组织缺血缺氧损伤,改善CPR后大鼠行为学及认知功能。
Objective: To investigate the effects of remote limb ischemic preconditioning (RLIP) training on the behavior and cognitive function of rats after cardiopulmonary resuscitation (CPR) to explore the protective effect of RLIP training on the neurological function of rats after CRP. Methods: Adult male Sprague-Dawley rats were randomly divided into four groups: Sham group, control group, RLIP group and RLIP training group. CPR model and RLIP model were not established in Sham group. CPR model was established by VF in control group. CPR was performed for 8 min, then CPR was started and ROSC was restored. RLIP group: 24-48 h before establishing CPR model remote limb ischemic preconditioning. RLIP training group: rats were subjected to continuous limb ischemic preconditioning training for 2 weeks to establish CPR model. The neurological deficit score (NDS) was recorded on the 1st day and the 3rd day after resuscitation in rats. The EN1, ST and EN2 scores of rats in ST-segment after resuscitation were recorded. Results: (1) NDS in control group, RLIP group and RLIP training group were significantly lower than those in Sham group (P <0.05). NDS at 1 and 3 days after CPR in RLIP group and RLIP training group were significantly better than those in control group P <0.05). There was no significant difference between RLIP group and RLIP training group (P> 0.05). (2) Compared with Sham group, the EN1 and EN2 values of control group, RLIP group and RLIP training group both increased and the ST time shortened (P <0.05). The learning and memory abilities of RLIP group and RLIP training group were significantly better than Sham group control group (P <0.05). There was no significant difference between RLIP group and RLIP training group (P> 0.05). CONCLUSION: RLIP training can reduce the hypoxic-ischemic injury of rat neural tissue after CPR and improve the behavior and cognitive function of rats after CPR.