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目的探讨活血定眩胶囊对CSA大鼠血液ET、NO、6-Keto-PGF_(1α)、TXB_2及椎动脉NF-κB表达的影响。方法 SPF级大鼠72只,随机分为空白组、模型组、颈复康组、活血定眩胶囊低剂量组、中剂量组、高剂量组等6组,采用植骨压迫+组织离断联合造模。造模成功后连续灌胃8周,检测血液ET、NO、6-Keto-PGF_(1α)、TXB_2及椎动脉NF-κB表达。结果与空白组相比,血液中ET、TXB_2浓度增加,而NO、6-Keto-PGF_(1α)浓度降低,椎动脉中NF-κB表达增强,差异有统计学意义(P<0.05);与模型组相比,活血定眩胶囊高剂量组ET、TXB_2浓度降低,而NO、6-Keto-PGF_(1α)浓度增加,差异有统计学意义(P<0.01),椎动脉中NF-κB表达减弱,差异有统计学意义(P<0.05),活血定眩胶囊中剂量组及颈复康组ET、TXB_2浓度降低,而NO、6-Keto-PGF_(1α)浓度增加,差异有统计学意义(P<0.05),椎动脉中NF-κB表达减弱,但差异无统计学意义(P>0.05),活血定眩胶囊低剂量组各指标值均无统计学意义(P>0.05)。与颈复康组相比,活血定眩胶囊高剂量组各指标值差异有统计学意义(P<0.05),活血定眩胶囊中、低剂量组各指标值差异均无统计学意义(P>0.05)。结论活血定眩胶囊能有效改善椎动脉型颈椎病模型大鼠血液ET、NO、6-Keto-PGF_(1α)、TXB_2的表达,明显抑制NF-κB的表达。
Objective To investigate the effects of Huoxuedinguo Capsule on the expression of ET, NO, 6-Keto-PGF_ (1α), TXB_2 and the expression of NF-κB in vertebral artery in CSA rats. Methods Seventy-two SPF rats were randomly divided into six groups: blank group, model group, Jinkuikang group, low-dose Huoxudingduo capsule group, middle-dose group and high-dose group. Modeling. After successful modeling, the rats were continuously gavaged for 8 weeks, and the expressions of ET, NO, 6-Keto-PGF_ (1α), TXB2 and NF-κB in the vertebral artery were detected. Results Compared with the blank group, the concentrations of ET and TXB_2 in the blood increased, while the concentrations of NO and 6-Keto-PGF_ (1α) decreased and the expression of NF-κB increased in the vertebral artery (P <0.05) Keto-PGF_ (1α) concentration in Huoxuedingzhuo capsule high-dose group was significantly lower than that in the model group (P <0.01), and the expression of NF-κB in vertebral artery (P <0.05). The concentrations of ET and TXB_2 in Huoxingdinguan capsule medium dose group and Jinkuikang group were decreased, while the concentrations of NO and 6-Keto-PGF_ (1α) were increased, the difference was statistically significant (P <0.05). The expression of NF-κB in vertebral artery was decreased, but the difference was not statistically significant (P> 0.05). There was no significant difference between the two groups (P> 0.05). Compared with Jinkuikang group, the indexes of Huoxingdingzhuo capsule high-dose group showed significant difference (P <0.05), but there was no significant difference between each index of Huoxuedinguan capsule and low-dose group (P> 0.05). Conclusion Huoxuedinglexuan capsule can effectively improve the expression of ET, NO, 6-Keto-PGF_ (1α) and TXB_2 in blood of rats with vertebral artery cervical spondylosis and obviously inhibit the expression of NF-κB.