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采用全细胞膜片钳技术研究颅痛定对酶解分离的豚鼠心室肌细胞膜钙通道电流(I_(Ca))的影响.结果:当保持电位为-40mV、去极化从-3O至+60mV、刺激脉宽为150ms、频率为1Hz时可记录到I_(Ca).维拉帕米10μmol/L可明显抑制I_(Ca).颅痛定1～100μmol/L可浓度依赖性抑制I_(Ca),10、30μmol/L分别使I_(Ca)峰值从-1135±224降至-832±216pA(n=7,P<0.01),从-1174±216降至-452±71PA(n=8,P<0.01),抑制百分率分别为27%、61%,冲洗后可部分恢复.提示颅痛定对I_(Ca)的抑制为其主要的抗心律失常作用机理之一.
Whole-cell patch-clamp technique was used to study the effects of cranialgia decoction on the calcium channel current (I_ (Ca)) in isolated guinea pig ventricular myocytes.When the holding potential was -40 mV and depolarization was from -3 to +60 mV, Stimulation of pulse width of 150ms, the frequency of 1Hz can be recorded to I_ (Ca). Verapamil 10μmol / L can inhibit I_ (Ca) , And 10,30μmol / L decreased the I Ca peak from -1135 ± 224 to -832 ± 216pA (n = 7, P <0.01), from -1174 ± 216 to -452 ± 71PA P <0.01), and the percent inhibition was 27% and 61%, respectively, which could be partially recovered after rinsing.It is suggested that the inhibition of cranial ache on I_ (Ca) is one of the main antiarrhythmic mechanisms.