论文部分内容阅读
目的:构建携带sTie2基因的重组慢病毒并研究其抑制结肠癌细胞成瘤的作用。方法:建立BALB/C小鼠结肠癌皮下成瘤模型,待到移植瘤肿瘤长至60mm3大小后,通过尾静脉注射重组慢病毒进行基因治疗2周后,处死小鼠并取下瘤组织检测其质量、体积,并包埋切片行免疫组化染色检测CD31的表达。结果:pLenti-sTie2抑制结肠癌小鼠移植瘤的生长及血管生成,并能有效促进肿瘤组织中Tie2的表达。差异均具有统计学意义。结论:pLenti-sTie2慢病毒可以显著抑制BALB/C结肠癌小鼠移植瘤内微血管的减少,从而抑制肿瘤增殖,为基因治疗结肠癌提供实验基础。
Objective: To construct a recombinant lentivirus carrying sTie2 gene and study its effect on inhibiting the tumorigenesis of colon cancer cells. METHODS: A subcutaneous tumor model of colon cancer of BALB/C mice was established. After the tumor of the transplanted tumor reached a size of 60 mm3, two weeks after gene therapy with recombinant lentivirus via the tail vein, the mice were sacrificed and the tumor tissue was removed to detect the tumor. Mass, volume, and embedded sections were used to detect the expression of CD31 by immunohistochemical staining. RESULTS: pLenti-sTie2 inhibited the growth and angiogenesis of colon cancer mice transplanted tumors, and could effectively promote the expression of Tie2 in tumor tissues. The differences were statistically significant. CONCLUSION: The pLenti-sTie2 lentivirus can significantly inhibit the reduction of microvessels in BALB/c colon cancer xenografts, thereby inhibiting tumor proliferation and provide experimental basis for gene therapy of colon cancer.