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目的 观察外周和中枢一氧化氮 (NO)在大鼠实验性变态反应性脑脊髓炎 (EAE)中的动态变化 ,探讨EAE大鼠发病的相关生物学机制。方法 采用免疫组化法和硝酸还原酶法 ,观察豚鼠全脊髓匀浆诱导的Wistar大鼠EAE的过程中 ,脊髓内表达iNOS胶质细胞与外周NO代谢物NO 2 和NO 3的变化。 结果 对照组脊髓内未发现表达iNOS阳性细胞 ,表达iNOS的CNS胶质细胞可能是小胶质细胞 ,而且它的变化与EAE大鼠的病情一致 ,评分 2分和 3分EAE大鼠脊髓表达iNOS的小胶质细胞比评分 1分大鼠明显增多 (P <0 .0 1) ,恢复期EAE大鼠表达iNOS的小胶质细胞明显减少 (P <0 .0 1)。EAE大鼠外周血清NO值随症状程度加重而升高 ,但在EAE恢复期时仍保持较高水平。未发病大鼠血清NO值明显增高 ,与对照组之间具有显著性差异 (P <0 .0 1)。 结论 小胶质细胞产生的NO可能在急性期EAE大鼠的发病中起重要作用
Objective To observe the dynamic changes of peripheral nitric oxide (NO) in rats with experimental allergic encephalomyelitis (EAE) and to explore the related biological mechanism of EAE. Methods Immunohistochemical method and nitrate reductase method were used to observe the changes of NO 2 and NO 3 in iNOS glial cells and peripheral NO metabolites in Wistar rats induced by whole spinal cord homogenate in guinea pigs. Results No iNOS positive cells were found in the spinal cord of the control group. The CNS glial cells expressing iNOS were probably microglia, and their changes were consistent with those of EAE rats. The scores of iNOS in spinal cord of 2 and 3 EAE rats (P <0.01). The number of microglial cells expressing iNOS in recovery EAE rats was significantly decreased (P <0.01). The serum NO level in peripheral blood of EAE rats increased with the exacerbation of symptoms, but remained high at the recovery of EAE. The serum NO level of the non-onset rats was significantly higher than that of the control group (P <0.01). Conclusion NO produced by microglia may play an important role in the pathogenesis of acute EAE rats