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目的:建立HPLC法测定肝康片中人参皂苷Rg_1和人参皂苷Re含量。方法:采用Diamonsil C_(18)柱(250 mm×4.6 nlm,5μm),以乙腈:0.05%磷酸(20:80)为流动相,检测波长:203 nm,流速1.0 ml·min~(-1)。结果:人参皂苷Rg_1、人参皂苷Re分别在0.424～4.24μg(r=0.999 9,n=5),0.964～9.64μg(r=0.999 7,n=5)范围内线性关系良好,平均加样回收率分别为99.92%(RSD=1.91%)和100.20%(RSD=1.83%)。结论:本法操作简便、结果准确、灵敏度高、重复性好,可用于肝康片中人参皂苷Rg_1和人参皂苷Re含量控制。
Objective: To establish an HPLC method for the determination of ginsenoside Rg_1 and ginsenoside Re in Gankang tablet. Methods: Diamonsil C_ (18) column (250 mm × 4.6 nlm, 5 μm) was used with mobile phase of acetonitrile 0.05% phosphoric acid (20:80) at the wavelength of 203 nm and the flow rate of 1.0 ml · min -1 . Results: Ginsenoside Rg_1 and ginsenoside Re showed a good linearity in the range of 0.424 ~ 4.24μg (r = 0.999 9, n = 5) and 0.964 ~ 9.64μg (r = 0.999 7, n = 5) Rates were 99.92% (RSD = 1.91%) and 100.20% (RSD = 1.83%), respectively. Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used to control the content of ginsenoside Rg_1 and ginsenoside Re in Hekang Tablets.