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目的 探讨大肠癌患者淋巴结转移的临床意义。方法 用逆转录聚合酶链反应 (RT PCR)法检测了 49例大肠癌患者淋巴结中细胞角蛋白 2 0 (CK 2 0 )mRNA的表达情况。结果 49例大肠癌标本中共检测淋巴结 34 2枚 ,常规病理检查和CK 2 0mRNA检测淋巴结转移的检出率分别为12 .6 %和 2 3.1% ,二者差异有显著性 (P <0 .0 1)。结论 检测大肠癌患者淋巴结CK 2 0mRNA的表达 ,可以提高大肠癌淋巴结转移的检出率 ,对大肠癌的准确分期、治疗方案的选择及预后的判断具有指导意义。
Objective To explore the clinical significance of lymph node metastasis in patients with colorectal cancer. Methods Reverse transcription polymerase chain reaction (RT PCR) was used to detect the expression of cytokeratin 20 (CK 2 0) mRNA in the lymph nodes of 49 patients with colorectal cancer. Results A total of 342 lymph nodes were detected in 49 specimens of colorectal cancer. The detection rate of lymph node metastasis in routine pathological examination and CK 2 0 mRNA was 12.6% and 2 3.1%, respectively. There was a significant difference between them (P < .0 1). Conclusion Detecting the expression of CK 2 0 mRNA in lymph nodes of patients with colorectal cancer can increase the detection rate of lymph node metastasis of colorectal cancer. It has guiding significance for the accurate staging of colorectal cancer, the choice of treatment plan and the judgment of prognosis.