建立测定微乳中水飞蓟素含量的反相高效液相色谱法。采用ODSC18色谱柱,检测波长为288nm,流动相为甲醇-水-冰醋酸(42:58:0.5,v/v/v),流速为1mL/min。水飞蓟宾标准曲线的线性范围为10-1000μg/mL,平均回收率为99.0%-100.7%,日内和日间精密度均低于1.6%。该法简单、快速、精确、准确,可用于水飞蓟素微乳制剂质量控制要求。 A reversed-phase high performance liquid chromatographic method was established for the determination of silymarin in microemulsion. An ODSC18 column was used. The detection wavelength was 288 nm. The mobile phase was methanol-water-glacial acetic acid (42:58:0.5, v/v/v) at a flow rate of 1 mL/min. The linear range of the standard curve of Silybin was 10-1000 μg/mL, the average recovery was 99.0%-100.7%, and the intra-day and inter-day precision were both below 1.6%. The method is simple, rapid, accurate and accurate and can be used for the quality control requirements of silymarin microemulsion formulations.