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目的观察提前给予小鼠叶黄素干预对砷肝脏毒性的预防效果。方法将84只SPF级昆明种小鼠,雌雄各半,随机分为35 d空白组、70 d空白组、35 d单纯叶黄素组、70 d单纯叶黄素组、砷染毒组、35 d叶黄素拮抗组和70 d叶黄素预防组,按叶黄素40 mg/kg,As2O35 mg/kg剂量灌胃给药。测定血清中谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性,肝脏匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)含量、总抗氧化能力(T-AOC)及一氧化氮(NO)含量,采用单因素方差分析对比组间差异。结果砷染毒组AST和ALT活性、MDA含量显著高于70 d空白组,SOD活性、GSH及NO含量、T-AOC水平显著低于70 d空白组(P<0.05);70 d叶黄素预防组AST及ALT活性、MDA含量低于35 d叶黄素拮抗组(P<0.05),SOD活性、NO及GSH含量、T-AOC水平均高于35 d叶黄素拮抗组(P<0.05)。结论提前给予叶黄素干预可以提高小鼠肝脏组织抗氧化损伤能力,从而拮抗砷的肝脏毒性作用。
Objective To observe the preventive effect of lutein intervention on arsenic liver toxicity in mice. Methods Eighty-four SPF Kunming mice were randomly divided into three groups: 35 days blank group, 70 days blank group, 35 days simple lutein group, 70 days simple lutein group, arsenic exposure group, 35 d lutein antagonist group and 70 d lutein prevention group, according to lutein 40 mg / kg, As2O35 mg / kg dose administration. Serum glutamate aminotransferase (ALT), aspartate aminotransferase (AST) activity, liver homogenate malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, reduced glutathione (GSH), total antioxidant capacity (T-AOC) and nitric oxide (NO) were measured by one-way ANOVA. Results The levels of AST and ALT, the content of MDA in arsenite-treated group were significantly higher than those in the control group at 70 d (P <0.05). The levels of SOD, GSH, NO and T-AOC in arsenite- The activities of AST and ALT in the prophylaxis group were lower than those in the lutein group (P <0.05), and the levels of SOD, NO and GSH and T-AOC in the prevention group were higher than those in the lutein group (P <0.05) ). Conclusion Lutein pretreatment can increase the anti-oxidative damage ability of liver tissue in mice and thus antagonize the toxic effects of arsenic on liver.