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目的探讨降纤酶对局灶性脑缺血再灌流损伤的保护作用。方法用线栓法制成大鼠大脑中动脉闭塞 再灌流模型 ,1 82只大鼠分为 :正常对照组 ;假手术组 ;缺血 3h再灌流 (3h、6h、2 4h、72h) ,缺血 6h再灌流 (3h、6h、2 4h)组 ,缺血再灌注组分为治疗组 :再灌流前 30min尾静脉注射降纤酶 (8U Kg) ,对照组 :静脉注射等容量生理盐水。行大鼠神经功能评分 ;脑组织TTC染色 ,计算梗塞灶体积 ;HE染色观察脑组织病理变化。结果缺血 3h再灌注大鼠降纤酶治疗组各时间点神经功能评分均较对照组低 (P <0 .0 5) ,梗死灶体积较对照组小 (P <0 .0 5)。缺血 6h再灌注组降纤酶治疗组的神经功能评分和梗死灶体积与对照组比较无显著性差异 (P>0 .0 5)。降纤酶组未见脑内出血加重的倾向。结论降纤酶对大鼠局灶脑缺血 3h再灌流引起神经功能障碍和脑组织损伤有保护作用。
Objective To investigate the protective effect of defibrase on focal cerebral ischemia-reperfusion injury. Methods Rat middle cerebral artery occlusion (MCAO) reperfusion model was established by thread occlusion. One hundred and eighty-two rats were divided into four groups: normal control group, sham operation group, ischemia and reperfusion 3h, 6h, 24h and 72h, 6 h reperfusion (3 h, 6 h, 24 h). The rats in ischemia-reperfusion group were treated with defibrase (8U Kg) in the tail vein 30 min before reperfusion, and the control group was injected with the same volume of normal saline. The neurological function scores of rats were obtained; TTC staining of brain tissue was used to calculate infarction volume; HE staining was used to observe the pathological changes of brain tissue. Results Compared with the control group, the scores of neurological function of the defibrase-treated group were lower than that of the control group (P <0.05). The volume of the infarct lesion was smaller than that of the control group (P <0.05). There was no significant difference in neurological function scores and infarct size between the 6-h reperfusion group and the control group (P> 0.05). Defibrase group no tendency to increase bleeding in the brain. Conclusion Defibrase has a protective effect on neurological dysfunction and brain injury caused by reperfusion after focal cerebral ischemia 3h in rats.