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水果和蔬菜中含有的有机酸,称谓果酸,果酸的定量方法很多,气相色谱法需要将果酸衍生后才能分析。本文阐述用反相高效液相色谱及紫外与示差折光检测器,流动相为0.01M(NH_4)_2HPO_4,用H_3PO_4调节pH值为2.5,使果酸得到分离。用保留时间定性,用标准酸样的直线方程进行定量。并对橙子中的柠檬酸、健力宝饮料中的酒石酸作了定量测定。回收率达90%以上。此法快速,分离效果较好,是食品生产与分析发展的趋势。
Fruits and vegetables contain organic acids, appellation of acid, acid quantitative methods, gas chromatography need to derive the acid can be analyzed. This article describes the use of reverse phase high performance liquid chromatography and UV and refractive index detector, the mobile phase 0.01M (NH 4) _2HPO_4 H_3PO_4 with the pH adjusted to 2.5, the acid was separated. Qualitatively, retention time was quantified using a standard acid-based linear equation. And orange citric acid, Jianlibao tartaric acid in beverages for quantitative determination. Recovery rate of 90% or more. This method is fast, the separation effect is better, is the trend of food production and analysis.