A novel type of molecular tweezers with different chiral center and cleft has been designed and prepared by usingα-hyodeoxycholic acid as spacer and D/L-amino acid methyl ester as chiral arm attached at 3-position.Their structures were elucidated by ~1H NMR,FTIR and elemental analysis.Their recognition properties for various D/L-amino acid methyl esters were also investigated.The preliminary results indicated that these chiral single-armed molecular tweezers exhibited good recognition ability for D/L-amino acid methyl esters with high association constant(up to 5.24×10~3 L mol~(-1)). A novel type of molecular tweezers with different chiral center and cleft has been designed and prepared by using α-hyodeoxycholic acid as spacer and D / L-amino acid methyl ester as chiral arm attached at 3-position. Their structures were elucidated by ~ 1 H NMR , FTIR and elemental analysis. The recognition properties for various D / L-amino acid methyl esters were also investigated. The preliminary results indicated that these chiral single-armed molecular tweezers exhibited good recognition ability for D / L-amino acid methyl esters with high association constant (up to 5.24 × 10 ~ 3 L mol -1).