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背景与目的:survivin是一种凋亡抑制基因,在肿瘤发生和转归中起重要作用。本研究探讨survivin基因启动子区域-31G/C多态性与胃癌及survivinmRNA表达的关系。方法:采用聚合酶链反应-限制性片段长度多态性分析技术(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)检测96例胃癌患者癌组织和67例正常人外周血中的survivin基因多态性,同时用实时定量逆转录聚合酶链反应(real-time quantitative reverse transcription-polymerase chain reaction,RQ-RT-PCR)对肿瘤组织的survivin mRNA进行了定量检测。结果:胃癌患者survivin-31位GG、GC和CC基因型频率为20.84%、39.58%和39.58%,而正常对照组则分别为46.26%、41.80%和11.94%。胃癌患者-31C等位基因和C/C基因型频率分别为59.37%和39.58%,显著高于对照组的32.84%和11.94%[χ2=22.26,P<0.005,OR=2.98,95%CI=1.96～4.51;χ2=14.88,P<0.005,OR=4.83,95%CI=2.91～8.03]。胃癌组织高表达survivin mRNA,但其表达强度在各基因型之间无显著性差异。结论:survivin基因启动子-31C/C基因型和C等位基因与胃癌有关,可能是肿瘤发生的易感因素之一。
BACKGROUND & OBJECTIVE: Survivin is an apoptosis-inhibiting gene that plays an important role in tumorigenesis and prognosis. This study was to investigate the relationship between -31G / C polymorphism of survivin gene promoter region and the expression of survivin mRNA and gastric cancer. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the expression of survivin gene in 96 cases of gastric cancer and 67 cases of normal controls Meanwhile, the expression of survivin mRNA in tumor tissue was detected by real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR). Results: The frequencies of survivin-31 GG, GC and CC genotypes in gastric cancer patients were 20.84%, 39.58% and 39.58%, respectively, compared with 46.26%, 41.80% and 11.94% in normal controls. The frequencies of -31C allele and C / C genotype in patients with gastric cancer were 59.37% and 39.58% respectively, significantly higher than 32.84% and 11.94% in the control group [χ2 = 22.26, P <0.005, OR = 2.98, 95% CI = 1.96 ~ 4.51; χ2 = 14.88, P <0.005, OR = 4.83, 95% CI = 2.91 ~ 8.03]. Survivin mRNA was highly expressed in gastric cancer tissues, but the expression intensity of survivin mRNA was not significantly different between the two genotypes. Conclusion: The gene of survivin promoter -31C / C and C allele are associated with gastric cancer, which may be one of the predisposing factors for tumorigenesis.