论文部分内容阅读
目的为了克服基因枪接种乙型肝炎表面抗原(HBsAg)DNA疫苗诱生的免疫应答以Th2为主的缺点,在基因枪接种质粒HBsAg DNA疫苗的同时共导入或共表达乙型肝炎病毒壳(HBV core)基因作为佐剂,以促进其所诱生的HBsAg特异性的Th2型免疫应答向Tn1型转换。方法构建可单独或共同表达HBsAg或核心抗原(HBcAg)的DNA免疫用载体pIRKS／core、pIRES／C149、pIRES／S、pIRES／S／Core和pIRES／S／C149,并在真核细胞进行表达验证。对BALB／c雌鼠进行免疫并检测小鼠免疫后的特异性体液免疫和细胞免疫指标。结果共导入或共表达HBV core基因能增强基因枪接种HBsAg DNA疫苗诱生的Th1型免疫应答水平,包括HBsAg特异的IgG2a应答、CTL活性、IFN-γ产生能力等。结论以HBV core基因为佐剂能促进基因枪接种HBsAg DNA疫苗诱生的Th2型免疫应答向Th1型免疫应答转换。
In order to overcome the shortcoming of predominant Th2-induced immune response induced by DNA vaccine against hepatitis B surface antigen (HBsAg), the HBsAg DNA vaccine was introduced or co-expressed in Hepatitis B virus (HBV) core gene as an adjuvant to facilitate the conversion of the HBsAg-specific Th2-type immune response into Tn1 type. Methods The DNA immunization vectors pIRKS / core, pIRES / C149, pIRES / S, pIRES / S / Core and pIRES / S / C149 that can express HBsAg or core antigen (HBcAg) alone or in combination were constructed and expressed in eukaryotic cells verification. The BALB / c female mice were immunized and the specific humoral and cellular immune indices after immunization were tested. Results The introduction or co-expression of HBV core gene could enhance the level of Th1 immune response induced by HBsAg DNA vaccine, including HBsAg-specific IgG2a response, CTL activity and IFN-γ production ability. Conclusion Using HBV core gene as an adjuvant can promote the conversion of Th2 immune response induced by HBsAg DNA vaccine to Th1 immune response.