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硫胺无燐光,在碱中氧化后才产生燐光。 λ激发=300nm,λ发射=410 nm,燐光寿命为1.3s.硫胺燐光受溶剂极性影响甚小,15种金属离子中只有铜离子可增强流胺燐光,寿命变短为 0. 94 s,但不影响燐光光谱。在开始30min内有CuSO4的作用较快,以后逐渐变慢,到 4h后达平衡与不用CuSO4的燐光相近,启示CuSO4具有催化硫胺的氧化作用,建立了硫胺的燐光分析方法,用于两种维生素B1片的分析,分别为98.5%与98%。
Sulfuric acid without calendering, catalase produced in the alkali oxidation. λ excitation = 300 nm, λ emission = 410 nm, and calendaring lifetime 1.3 s. Sulfuric acid amine light is very little affected by the polarity of the solvent, only 15 of the metal ions can enhance the flow of silver halide, life expectancy shortened to zero. 94 s, but does not affect the calendering spectrum. In the first 30 min, the effect of CuSO4 was faster and then gradually slowed down. After 4h, the equilibrium reached the same as that of CuSO4 without CuSO4, which suggested that CuSO4 could catalyze the oxidation of thiamine. Analysis of vitamin B1 tablets, respectively, 98.5% and 98%.