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采用拮抗试验、酯酶同工酶和分子标记对引进7个黑龙江主栽黑木耳菌株进行遗传差异分析。结果表明,在黑木耳遗传差异性分析中,3种方法具有一致性。通过酯酶同工酶与分子标记聚类分析,均能将来自于黑龙江同一地域的黑木耳分为4个类别:黑威9号与981遗传距离最近为第一类,黑威10号、8808与第一类2个菌株遗传距离较近为第二类,黑29与第一类、第二类4个菌株遗传距离较远为第三类,而9809、H916与以上5个菌株遗传距离最远为第四类。同时在拮抗试验中黑29、9809、H916三个遗传距离较远的菌株也表现出明显的拮抗现象。研究结果为黑木耳新品种引选以及种质资源评价分析提供技术支撑。
Antagonistic test, esterase isozymes and molecular markers were used to analyze the genetic diversity of seven main black fungus strains introduced from Heilongjiang Province. The results showed that the three methods were consistent in the genetic diversity analysis of black fungus. Through the analysis of esterase isozyme and molecular marker cluster analysis, the black fungus from the same area in Heilongjiang can be divided into four categories: the genetic distance between Heiwei 9 and 981 is the first type recently, Heiwe 10, 8808 The genetic distance to the two strains of the first type was close to the second type. The genetic distance between the black type 29 and the first type and the second type was far from that of the third type. The genetic distance between the strains 9809 and H916 and the above five strains was the highest Far for the fourth category. At the same time, antagonistic test black 29,9809, H916 three genetic distance far strains also showed obvious antagonism. The results provide technical support for introduction of new varieties of black fungus and evaluation of germplasm resources.