软化型氟骨症大鼠骨密度和骨生物力学的变化及硼预防的影响

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目的 :观察及分析软化型氟骨症大鼠骨密度 (BMD)和骨生物力学特性的变化及硼预防对此的影响。方法 :SD大鼠分为对照组、低过量氟组、高过量氟组和硼预防组 ,实验 2 0周后测量大鼠BMD和血清氟、硼含量、四肢骨氟含量及进行股骨生物力学检测。结果 :低过量氟组和高过量氟组大鼠血清游离氟、骨氟含量显著增高 ;全身和胫骨BMD降低 ,腰椎BMD变化不大 ;股骨最大负荷减低 ,最大形变增加。相比高过量氟组 ,硼预防组大鼠血清游离氟和骨氟含量明显下降 ;全身、腰椎和胫骨BMD显著增高 ;股骨最大负荷明显增加 ,最大形变明显减小。 4组大鼠血清硼含量无显著性差异。结论 :软化型氟骨症大鼠骨密度和骨生物力学特性变化明显 ,呈现骨软化和骨质疏松 ;饲料加硼对此变化有一定的预防作用 OBJECTIVE: To observe and analyze the changes of bone mineral density (BMD) and biomechanical properties in rats with softened osteoidrosis and the effects of boron prophylaxis. Methods: The SD rats were divided into control group, low excess fluoride group, high fluoride group and boron prevention group. After 20 weeks of experiment, the contents of serum and fluoride, boron and fluorine in the extremities were measured and the femoral biomechanical test . Results: Serum free fluoride in the rats in the low and high fluoride groups was significantly higher than that in the fluoride group. The BMD of the whole body and the tibia decreased, while the BMD of the lumbar spine did not change much. The maximum load of the femur decreased and the maximum deformation increased. Compared with the high fluoride group, the levels of free fluorine and bone fluoride in the serum of the B group decreased significantly; the BMD of the whole body, lumbar spine and tibia were significantly increased; the maximum load of the femur significantly increased and the maximum deformation was significantly reduced. Four groups of rats serum boron content was no significant difference. CONCLUSION: The bone mineral density and biomechanical properties of softened osteofctrosis rats are obviously changed, showing osteomalacia and osteoporosis. Boron supplementation may have a preventive effect on the changes
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