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目的:描述和分类自然发生的霍乱毒素B亚单位DNA和蛋白序列的多态性,及时提供潜在有用的霍乱诊断、疫苗研制和流行病学信息。方法:采用生物信息频谱分析平台,同时包括Clustal W,MEGA和PyMOL分析法,对245个霍乱毒素B亚单位DNA和蛋白序列进行结构和功能及基因分型特性的研究。结果:所分析的霍乱毒素B亚单位的103个氨基酸区域有共同的信息频谱特征,其主峰特征为F(0.3333,19),显示了霍乱菌株的霍乱毒素B亚单位的共同作用模式。霍乱毒素B亚单位蛋白的T68I氨基酸残基关键变异明显增加了F(0.3333)的特征峰高,可能对“人类”作用的模式有较大影响。经过系统聚类分析,发现了19个霍乱毒素B亚单位DNA序列多态。基于以上结果和流行病学资料,提出了19个霍乱弧菌的霍乱毒素B亚单位基因型。霍乱弧菌古典生物型参考株(569B)的霍乱毒素B亚单位基因型为1a(CTBgenotype 1a)。霍乱弧菌埃尔托生物型参考株(N16961)和霍乱弧菌O139血清群的参考株(4260B)的霍乱毒素B亚单位基因型都为2a(CTB genotype 2a)。结论:以上内容可对霍乱毒素B亚单位DNA和蛋白序列的多态性提供更好的理解;提供了对霍乱毒素B亚单位分子变异监测的一个技术和方案;可为今后预防和处置霍乱弧菌的感染和流行,进一步扩展了鉴别潜在的免疫、治疗和诊断的分子靶标的可能性。
PURPOSE: To describe and classify the naturally occurring DNA and protein sequence polymorphisms of the cholera toxin B subunit and provide timely and potentially useful information for the diagnosis, vaccine development and epidemiology of cholera. Methods: The structures, functions and genotyping characteristics of 245 cholera toxin B subunits DNA and protein sequences were studied by bioinformatics spectrum analysis platform, including Clustal W, MEGA and PyMOL analysis. RESULTS: The 103 amino acid regions of the cholera toxin B subunit analyzed shared a spectrum of information with a major peak characteristic of F (0.3333,19), indicating the mode of interaction of the cholera toxin B subunit of the cholera strain. The key mutation of T68I amino acid residues of cholera toxin B subunit protein significantly increased the characteristic peak height of F (0.3333), which may have a greater impact on the mode of “human” effect. After systematic cluster analysis, 19 cholera toxin B subunit DNA sequence polymorphisms were found. Based on the above results and epidemiological data, 19 cholera toxin B subunit genotypes were proposed. The cholera toxin B subunit genotype of Vibrio cholerae classical bio-reference strain (569B) was 1a (CTBgenotype 1a). The cholera toxin B subunit genotype of the V. cholerae Elto reference strain (N16961) and the reference strain (4260B) of the V. cholerae O139 serogroup was 2a (CTB genotype 2a). CONCLUSIONS: The above provides a better understanding of the polymorphisms in the DNA and protein sequences of the cholera toxin B subunit; provides a technique and protocol for monitoring the molecular variation of the cholera toxin B subunit; and may provide evidence for future prevention and treatment of cholera The infection and prevalence of bacteria further expand the possibility of identifying potential molecular targets for immunization, therapy and diagnosis.