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本研究以金钻蔓绿绒根茎、叶片、叶基、叶柄为试验材料,研究外植体类型与取材时间、消毒处理方法,植物激素种类与浓度筛选出适宜诱导愈伤组织、不定芽与继代培养基。结果表明:叶片和叶基最佳灭菌时间:75%酒精30 s+0.1%升汞10 min,叶柄:75%酒精30 s+0.1%升汞16 min,根茎:75%酒精30 s+0.1%升汞20 min;最佳取材时间为夏季,愈伤诱导率高,且诱导时间短;叶片、叶基、叶柄最佳诱导愈伤培养基:MS+2.0 mg/L TDZ+0.2 mg/L 2,4-D,增殖培养基:MS+3.0 mg/L 6-BA+0.2 mg/L NAA;根茎诱导不定芽及继代增殖最佳培养基分别为:MS+1 mg/L 6-BA+0.3 mg/L NAA+1 mg/L GA3与MS+0.8 mg/L 6-BA+0.2 mg/LIBA。
In this study, rhizomes, leaves, leaf bases and petiole of Acacia crassicarpa were used as experimental materials to study the types of explants, the time of their collection, the methods of disinfection, the types and concentrations of plant hormones, and the suitable callus induction, adventitious buds and succession Substitute for medium. The results showed that the best sterilization time of leaf and leaf was 75% alcohol 30 s + 0.1% mercuric chloride 10 min, petiole 75% alcohol 30 s + 0.1% mercuric chloride 16 min, rhizome 75% alcohol 30 s + 0.1 % Mercuric chloride for 20 min. The best induction time was summer, the callus induction rate was high, and the induction time was short. The best callus induction medium for leaf, leaf and petiole was MS + 2.0 mg / L TDZ + 0.2 mg / L 2,4-D and proliferation medium: MS + 3.0 mg / L 6-BA + 0.2 mg / L NAA. The best media for inducing adventitious buds and subculture were MS + 1 mg / L 6-BA +0.3 mg / L NAA + 1 mg / L GA3 and MS + 0.8 mg / L 6-BA + 0.2 mg / L IBA.