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目的：探讨阴道镜检查联合宫颈冷刀锥切术在诊治宫颈上皮内瘤变的临床价值。方法对2009年10月~2013年11月经门诊阴道镜下多点活检病理诊断为高级别宫颈上皮内瘤变(Ⅱ~Ⅲ级)48例患者行宫颈冷刀锥切术的临床资料进行分析探讨。结果宫颈冷刀锥切术后病理与阴道镜下多点活检结果一致者39例(81.25%)；不一致者9例(18.75%)，其中术后级别升高者7例(1例为宫颈早期浸润癌)，级别降低者2例。两种方法病理结果经统计学处理(<0.05)，差异有统计学意义。结论阴道镜检查联合宫颈冷刀锥切术可以提高宫颈上皮内瘤变的诊断准确率，这一方法为早期诊断宫颈癌的最佳组合，有推广应用价值。“,”Objective To explore the clinical value of colposcopy combined with cervical cold knife conization in diagnosis and treatment of cervical intraepithelial neoplasia. Methods From October 2009 to November 2013, 48 cases patients of colposcopy multipoint biopsy results for cervical intraepithelial neoplasia (CIN) II~Ⅲ were treated by cervical cold knife conization. Results Compared with pathological results between cervical cold knife conization and colposcopy multipoint biopsy, both results are consistent in 39 cases (81.25%), inconsistent in 9 cases (18.75%). Among them 7 cases of level elevated (1 case was diagnosised invasive cervical carcinoma), 2 cases of level lower. Pathological results of two methods were statistical y analyzed ( <0.05), the dif erence has statistical significance. Conclusion Colposcopy combined with cervical cold knife conization can improve the accuracy rate in the diagnosis of cervical intraepithelial neoplasia, this method was the best combination of early diagnosis of cervical cancer, and has a wide application value.