论文部分内容阅读
采用衍生化液相色谱-荧光检测法,研究了阿维菌素及甲氨基阿维菌素苯甲酸盐(甲维盐)在甲基咪唑存在下与三氟乙酸酐衍生化反应后样品溶液放置时间及保存温度对检测结果的影响。研究表明:在常温下,阿维菌素衍生化反应溶液放置180 min后、甲维盐衍生化反应溶液放置360 min后,两者衍生化产物的峰面积即急剧下降;衍生化反应20 min后,样品于4℃下保存(冷藏),阿维菌素在2 d内、甲维盐在3 d内,两者衍生化产物的检测结果保持稳定;而在-20℃下保存(冷冻),5 d内可保证阿维菌素和甲维盐衍生化后检测结果的准确性。因此在运用衍生化液相色谱荧光检测法时,应严格控制好衍生化反应后样品溶液的放置时间及保存温度,以保证检测结果的准确性。
The derivatized liquid chromatography-fluorescence detection method was used to study the effect of avermectin and emamectin benzoate (carbaryl) on the sample solution after derivatization with trifluoroacetic anhydride in the presence of methylimidazole Place the time and save the temperature on the test results. The results showed that at room temperature, the peak area of the derivatized products dropped sharply after 180 minutes of derivatization of avermectin for 180 min after the derivatization reaction solution was placed for 360 min. After derivatization reaction for 20 min , The samples were stored at 4 ° C (refrigerated), the avermectins within 2 days, the metformin within 3 days, the results of the two derivatization products remained stable, while the samples were stored at -20 ° C (frozen) 5 d to ensure the accuracy of the test results after derivatisation of abamectin and carbaryl. Therefore, the use of derivatization liquid chromatography fluorescence detection method should be strictly controlled after the derivatization reaction sample solution storage time and storage temperature to ensure the accuracy of test results.