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Katz等(1972)改良的加藤氏厚涂片法在实际应用时不易掌握其透明时间、难以识别粪便内的虫卵、过度透明可使卵壳和毛蚴轮廓不清,并有厚涂片容易干燥等缺点。本文报道了对方法进行改良的结果。取1ml粪样放在纸上,上覆一块3cm见方的尼龙纱,将纱揿入粪后用小棒刮取滤出的粪便,移放在有一特制塑料板的洁净玻片上。板的中央有一直径为7mm的凹井,填平此凹约需粪便4.3mg。移去板后,留下的粪样覆以一块事先在含有0.3%亚甲蓝的25%甘油中浸24小时的3.5cm×2.5cm的玻璃纸。然后翻转玻片,在一平面上压平,使粪均匀散开,置镜下计数并记录透明所需的时间。
Katz et al (1972) modified Kato’s thick smear in practice is not easy to grasp the transparent time, it is difficult to identify the eggs in the stool, excessive transparency can make the egg shell and miraculous outline, and thick smear easy to dry Other shortcomings. This article reports the results of an improved method. Take 1ml fecal sample on paper, covered with a piece of 3cm square nylon yarn, the gauze into the dung, use a small stick to scrape the leached feces, moved on a clean glass with a special plastic plate. The center of the plate has a diameter of 7mm concave wells, fill the concave need to be stool 4.3mg. After removing the plate, the remaining feces sample was covered with a piece of 3.5 cm x 2.5 cm cellophane previously soaked in 25% glycerol containing 0.3% methylene blue for 24 hours. The slide is then turned over, flattened on a flat surface, the feces are spread evenly, the mirror is counted and the time required for clarity is recorded.