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建立了离子液体双水相萃取结合柱前衍生测定青霉素G的检测方法。由离子液体1-丁基-3-甲基咪唑四氟硼酸盐[Bmim]BF_4和(NH_4)_2SO_4构成离子液体双水相体系萃取衍生后的青霉素G,并采用高效液相色谱对其进行检测。考察了离子液体与盐不同浓度组成对萃取效率的影响。结果表明,萃取率受[Bmim]BF_4和(NH_4)_2SO_4的浓度影响,当体系中[Bmim]BF_4的质量分数达到30%、(NH_4)_2SO_4的质量分数在24.72%-33.11%之间时,萃取率可达到93.21%±1.42%(n=6),定量下限(LOQ)为97μg/mL。这一体系能够有效地萃取并检测青霉素G,具有很高的灵敏度和萃取率。
The determination of penicillin G by ionic liquid aqueous two-phase extraction combined with pre-column derivatization was established. The derivatized penicillin G was extracted by ionic liquid aqueous two-phase system from ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate [Bmim] BF_4 and (NH_4) _2SO_4 and was subjected to high performance liquid chromatography Detection. The effects of different concentrations of ionic liquid and salt on the extraction efficiency were investigated. The results showed that the extraction rate was affected by the concentrations of [Bmim] BF_4 and (NH_4) _2SO_4. When the mass fraction of [Bmim] BF_4 reached 30% and the mass fraction of (NH_4) _2SO_4 was between 24.72% and 33.11% The extraction rate was 93.21% ± 1.42% (n = 6), and the limit of quantification (LOQ) was 97μg / mL. This system can effectively extract and test penicillin G with high sensitivity and extraction rate.