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采用亚洲棉渐渗的纤维强度突出的陆地棉优质新品系0-153与陆地棉转基因抗虫新品系sGK9708为亲本,构建了F2及F2:3分离群体。利用3869对SSR引物筛选亲本,得到125对多态性引物。进一步对183个F2群体单株分析得到150个多态性标记位点,其中100个标记位点连锁,构建20个连锁群,共覆盖660cM,占棉花总基因组的14.67%,每个连锁群平均包含5个标记位点,标记间平均相距6.6cM,其中13个连锁群确定了对应的染色体。利用F2和F2:3数据,通过复合区间作图,共检测到28个产量及相关因素的QTLs。这些控制产量性状的QTLs只存在于5个连锁群上,成簇分布。与皮棉产量性状有关的2个QTLs,均与其它多个产量相关性状的QTLs在同一个连锁区段内,增效基因遗传效应方向一致,有必要研究其在标记辅助选择中的效果。本研究没有检测到在多世代表现稳定的QTL。因此,需要培育重组自交系,进一步明确产量性状有关QTL的遗传效应。
F2 and F2: 3 segregation populations were constructed using a new highland cotton line 0-153 with high fiber intensity, and sGK9708, a new line of transgenic cotton with upland cotton. The parents were screened by 3869 pairs of SSR primers and 125 pairs of polymorphic primers were obtained. A total of 150 polymorphic marker loci were obtained from 183 individuals of F2 population. Among them, 100 loci were linked to construct 20 linkage groups, covering a total of 660 cM, accounting for 14.67% of the total cotton genome. Each linkage group averaged Including five marker sites, the average distance between markers was 6.6cM, of which 13 linkage groups identified the corresponding chromosome. Using F2 and F2: 3 data, 28 QTLs for yield and related factors were detected by compound interval mapping. These QTLs controlling yield traits only existed in five linkage groups and distributed in clusters. The two QTLs related to lint yield traits were in the same linkage region with QTLs of other yield-related traits. The synergistic genes had the same direction of genetic effect. It is necessary to study the effect of QTLs in marker-assisted selection. No QTLs that were stable in many generations were detected in this study. Therefore, it is necessary to cultivate recombinant inbred lines to further clarify the genetic effects of QTLs related to yield traits.