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米粒长、饭粒长和饭粒延伸系数等性状与米饭品质密切相关。以籼稻台中本地1号(TN1)与粳稻春江06为亲本构建的加倍单倍体群体为材料,利用全基因饱和分子标记连锁遗传图谱对多个稻米出饭特性相关的性状进行QTL定位,共检测到14个QTL。其中,米粒长和米粒浸长QTL各1个,均位于第2染色体上,分别可以解释性状变异的15.20%和18.50%;1个米粒浸泡膨胀率QTL,位于第6染色体上,可解释性状变异的13.39%;1个煮饭粒长QTL,位于第9染色体上,可解释性状变异的13.60%;3个蒸饭粒长QTL,分别位于第1、3和12染色体上,共解释性状变异45.00%;4个煮饭延伸率QTL,分别位于第3、6、9和10染色体上,共解释性状变异61.30%;3个蒸饭延伸率QTL分别位于第1、3和6染色体上,共解释性状变异49.10%。在已知的Wx和ALK基因所在区域都检测到了米饭延伸性相关的QTL。相比较而言,覆盖ALK基因的QTL对出饭特性的影响更大,LOD值达到了6.35。该研究结果可为稻米出饭特性相关调控基因的克隆奠定基础,同时对稻米品质的改良及高产优质稻品种的分子标记辅助选育提供理论参考。
Rice grain length, grain length and rice grain extension coefficient and other properties are closely related to rice quality. Using doubled haploid population from indica Taichung Local 1 (TN1) and Japonica Chunjiang 06 as parents, the QTLs for traits related to rice performance were detected by using full-length gene-linked molecular marker linkage map. To 14 QTLs. Among them, one QTL for rice grain length and rice leaching length were located on chromosome 2, which could account for 15.20% and 18.50% of variance respectively. One QTL for soaking swelling rate on rice chromosome 6 explained the variation of trait 13.39% of the total rice grain length. One QTL for grain length was located on chromosome 9, accounting for 13.60% of the variance in grain size. Three QTLs for rice length were located on chromosomes 1, 3 and 12, respectively. Four QTLs for rice elongation were located on chromosomes 3, 6, 9 and 10 respectively, which explained 61.30% of the total variation. QTLs for elongation of three rice dishes were located on chromosomes 1, 3 and 6 respectively, which explained the traits Variation 49.10%. Rice extension-related QTLs were detected in the region of known Wx and ALK genes. In contrast, QTLs that cover the ALK gene have a greater impact on food performance, with a LOD value of 6.35. The results of this study can lay a foundation for the cloning of regulation genes related to rice properties, and provide theoretical references for the improvement of rice quality and the molecular marker-assisted breeding of high-yielding and high-yielding rice varieties.