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目的 观察视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍ ｅｎｔｅｐｉｔｈｅｌｉｕｍ ，ＲＰＥ）细胞对体外抗原特异性活化淋巴细胞的影响，探索ＲＰＥ细胞在眼免疫赦免中的作用。 方法 建立ＲＰＥ与抗原特异性Ｔ淋巴细胞系和静止淋巴细胞之间的共培养系统。使用基因组ＤＮＡ电泳、ＤＮＡ末端标记和流式细胞技术检测凋亡的诱导情况。 结果 与ＲＰＥ共培养的抗原特异性活化淋巴细胞出现凋亡征象。随着时间的延长，凋亡细胞的数量逐渐增加。定量分析表明：共育２４小时的凋亡细胞占５．９５％ ；４８小时占９．３８％ ；７２小时占１７．９５％ 。与此相反，ＲＰＥ对于静止的Ｔ细胞则不具有显著诱导凋亡的作用。 结论 ＲＰＥ细胞具有诱导入侵淋巴细胞凋亡的能力。这种现象作为免疫反应的限制机制，在眼后节免疫赦免的维持和保护眼免受免疫性炎症反应中可能具有重要的意义。
Objective To investigate the effect of retinal pigment epithelium (RPE) cells on antigen-specific activation of lymphocytes in vitro and to explore the role of RPE cells in immunopharmaceutical immunotherapy. Methods Establishment of co-culture system between RPE and antigen-specific T lymphocyte and resting lymphocytes. The induction of apoptosis was detected using genomic DNA electrophoresis, DNA end labeling and flow cytometry. Results Antigen-specific activated lymphocytes co-cultured with RPE showed signs of apoptosis. With the extension of time, the number of apoptotic cells gradually increased. Quantitative analysis showed that: 24 hours of co-incubation of apoptotic cells accounted for 5.95%; 48 hours accounted for 9.38%; 72 hours accounted for 17.95%. In contrast, RPE does not have a significant apoptosis-inducing effect on quiescent T cells. Conclusion RPE cells have the ability of inducing apoptosis of lymphocytes. This phenomenon, as a limiting mechanism of immune response, may have important implications for the maintenance and protection of the eye against immunological inflammatory responses in the posterior segment of the eye.