目的利用近红外透反射光谱技术,对复方苦参注射液渗漉液中多组分的量进行快速测定。方法采用氧化槐果碱、氧化苦参碱的HPLC测定值,总糖的苯酚-硫酸法测定值及固体总量的烘烤法测定值为对照值,用偏最小二乘法建立近红外光谱与对照值之间的校正模型,并对未知样品进行定量预测。结果校正模型对氧化槐果碱、氧化苦参碱、总糖和固体总量4种组分的交叉验证均方根误差分别为43.5、135.4、1 255.7 mg/L和150.7 mg/mL,相关系数分别为0.926 6、0.954 6、0.953 9和0.974 1。经外部验证,预测均方根误差分别为32.5、191.7、1 461.9 mg/L和164.0 mg/mL。结论本方法方便、准确、可靠,可用于中药渗漉过程的快速分析。 Objective To determine the amount of multi-component in percolate of Compound Kushen Injection by near-infrared transmission spectroscopy. Methods Oxidation Sophocine, oxymatrine HPLC determination, the total sugar phenol - sulfuric acid determination of the total amount of the measured value of the baking method as a control value, using partial least squares method to establish near-infrared spectroscopy and control Calibration model between the values, and quantitatively predict unknown samples. The results showed that the root mean square error of the calibration model was 43.5, 135.4, 1 255.7 mg / L and 150.7 mg / mL, respectively, and the correlation coefficients of cross-validation of 4 components of oxycinnamine, oxymatrine, total sugar and total solids Respectively 0.926 6,0.954 6,0.953 9 and 0.974 1. The external validation showed that the root mean square error of prediction was 32.5, 191.7, 1 461.9 mg / L and 164.0 mg / mL, respectively. Conclusion The method is convenient, accurate and reliable and can be used for the rapid analysis of percolation process of Chinese medicine.