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2008—2012年广西海洋研究所海水增养殖基地暂养的拟穴青蟹膏蟹出现“嗜睡”等典型的青蟹呼肠孤病毒病症状。采用套式PCR检测濒死膏蟹感染青蟹呼肠孤病毒阳性率为100%,经Blastn比对扩增序列与青蟹呼肠孤病毒基因(HQ414137.1)相似度达98%,属于青蟹呼肠孤病毒(JX213634)。病毒粗提液人工回感后发现,攻毒1h,青蟹血淋巴中即可检测到青蟹呼肠孤病毒;攻毒后第4～6d,出现死亡高峰,第10d,累积死亡率达到100%。血清酶指标中碱性磷酸酶、谷丙转氨酶和谷草转氨酶活力明显高于对照组,而与免疫相关的过氧化物酶、超氧化物歧化酶活力低于对照组;肝胰腺、鳃和肌肉组织切片均呈现显著病理坏死损伤。青蟹呼肠孤病毒是此次拟穴青蟹膏蟹死亡的主要原因。
In 2008-2012, the symptoms of typical blue crab reovirus disease such as “drowsiness” appeared in the crested crab crabs raised by the Guangxi Zhuang Autonomous Region Institute of Marine Research in the Seawater Culture Breeding Base. The nested PCR method was used to test the positive rate of reovirus infected by B. morulae to 100%. The similarity between the amplified sequence and the blue-crab reovirus gene (HQ414137.1) by Blastn was 98% Crab reovirus (JX213634). Virus crude extract after artificial back found, attack 1h, the crab can be detected in the crab spleen crab reovirus; attack after the first 4 ~ 6d, the peak of death, the first 10d, the cumulative mortality rate of 100 %. The activity of alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase of serum enzymes were significantly higher than those of the control group, while the activity of peroxidase and superoxide dismutase related to immunity were lower than that of the control group. The activities of hepatopancreas, gill and muscle tissue Slices showed significant pathological necrosis. Blue crab reovirus is the main reason for the crab crab crab death.