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目的 :研究T淋巴细胞核仁形成区嗜银蛋白 (Ag NORs)在原发性肝癌免疫学诊断中的意义。方法 :采用KL型肿瘤免疫计算机图像分析系统 ,测定 38例正常人和 2 5例原发性肝癌患者外周血T淋巴细胞Ag NORs的含量 ,以细胞核仁银染面积与细胞核面积比值 (I .S % )作为检测评估指标 ,以了解原发性肝癌患者Ag NORs的变化情况。同时 ,检测 2 5例原发性肝癌患者血AFP含量 ,以比较Ag NORs和AFP诊断原发性肝癌的灵敏性。结果 :2 5例原发性肝癌的平均I .S %值为 5 5 1%± 0 85 % ,明显低于正常人的 7 12 %± 0 78% ,二者差异显著 (P <0 0 5 ) ;Ag NORs的检测阳性率达 88% ,高于AFP的检测阳性率的 84% ,但差异无显著性 (P >0 0 5 )。 4例AFP正常的原发性肝癌患者中有 3例Ag NORs检测为异常。结论 :T淋巴细胞Ag NORs与原发性肝癌的诊断呈良好的相关性和较高的灵敏性 ,可望成为一种原发性肝癌免疫学诊断的指标
Objective: To study the significance of AgNORs in the nucleolar forming region of T lymphocytes in the immunological diagnosis of primary hepatocellular carcinoma. Methods: The KL tumor immune computer image analysis system was used to determine the content of Ag NORs in peripheral blood T lymphocytes in 38 normal subjects and 25 primary liver cancer patients. %) As a test evaluation indicator to understand the changes of Ag NORs in patients with primary liver cancer. At the same time, the level of blood AFP was detected in 25 patients with primary liver cancer to compare the sensitivity of Ag NORs and AFP in the diagnosis of primary liver cancer. Results: The average I.S.% of 25 cases of primary hepatocellular carcinoma was 5 1%±0 85%, which was significantly lower than that of normal individuals (7 12%±0 78%). There was a significant difference between the two groups (P <0 0 5 The positive rate of Ag NORs was 88%, which was higher than that of AFP, which was 84%, but the difference was not significant (P > 0.05). Three of the 4 patients with normal AFP with primary liver cancer had abnormal Ag NORs. Conclusion : Ag NORs in T lymphocytes have good correlation and high sensitivity in diagnosis of primary hepatocellular carcinoma. It is expected to be an indicator of immunological diagnosis of primary hepatocellular carcinoma.