植物名称:连翘(Forsythia Suspensa)。材料类别:子叶、苗端、下胚轴。取成熟的连翘种子,进行常规消毒后,接种于不加激素的MS培养基上。当苗高1.5—2cm时,在无菌条件下切取子叶、苗端、下胚轴分别接种。培养条件:以MS培养基为基本培养基,添加不同的激素,组成不同激素水平的培养基进行试验。在室温(17—23℃)黑暗条件下诱导愈伤组织,在26℃左右,每日光照10—12小时(光强为2000 Plant name: Forsythia Suspensa. Material categories: cotyledons, seedlings, hypocotyls. Forsythia forsythia seed taken, after routine disinfection, inoculated on hormone-free MS medium. When the seedling height 1.5-2cm, under aseptic conditions, cut cotyledons, seedlings, hypocotyls were inoculated. Culture conditions: MS medium as the basic medium, add different hormones, composed of different hormone levels in the medium for testing. Callus was induced under dark conditions at room temperature (17-23 ° C) with daily light at about 26 ° C for 10-12 hours (light intensity was 2000