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目的研究孕妇足月胎盘上人膜联蛋白Ⅴ(HAV)及β2糖蛋白Ⅰ(β2GPI)的表达变化,并探讨其在新生儿乙型肝炎病毒(HBV)宫内感染胎盘组织中的意义,从细胞分子水平角度探讨HBV宫内感染发生的机制。方法选取2013年7月-2014年1月在南昌大学第二附属医院妇产科分娩的血清乙肝表面抗原(HBsA g)阳性且肝功能正常、无其他任何妊娠并发症与合并症的40例单胎足月孕妇为研究对象,采用Elivision Plus/HRP免疫组化方法检测孕妇胎盘HAV、β2GPI蛋白,另选取20例HBV正常孕妇胎盘为对照组。结果血HBsA g阳性孕妇足月胎盘组织中HAV及β2GPI蛋白的表达明显高于对照组(P<0.05),且HAV及β2GPI蛋白在HBV DNA阳性组胎盘中的表达明显高于HBV DNA阴性组胎盘中的表达(P<0.05)。HAV与β2GPI蛋白在外周血HBsA g阳性孕妇足月胎盘组织中的表达呈正相关(r=0.538,P<0.05)。结论 HAV、β2GPI蛋白在HBsA g阳性胎盘组织中的表达明显上调,且两者呈正相关,两种蛋白在HBV宫内感染的发生、发展中起协同作用。
Objective To investigate the expression of human annexin Ⅴ and β2 glycoprotein Ⅰ in term placenta of pregnant women and to explore its significance in placenta of neonates with intrauterine infection of hepatitis B virus. To investigate the mechanism of HBV intrauterine infection at the cellular and molecular level. Methods From July 2013 to January 2014, 40 cases of hepatitis B surface antigen (HBsA g) positive and normal liver function were delivered in obstetrics and gynecology department of the Second Affiliated Hospital of Nanchang University without any other pregnancy complications and complications Fetal full-term pregnant women as the research object, the placenta HAV, β2GPI protein were detected by Elivision Plus / HRP immunohistochemical method, and 20 normal HBV placenta of pregnant women as the control group. Results The expression of HAV and β2GPI protein in full-term placenta of blood HBsA positive pregnant women was significantly higher than that of the control group (P <0.05), and the expression of HAV and β2GPI protein in the placenta of HBV DNA positive group was significantly higher than that of the HBV DNA negative placenta (P <0.05). There was a positive correlation between the expression of HAV and β2GPI protein in full-term placenta of HBsA g positive pregnant women (r = 0.538, P <0.05). Conclusions The expression of HAV and β2GPI protein in HBsAg-positive placenta is significantly up-regulated, and the two are positively correlated. The two proteins play a synergistic role in the pathogenesis and development of HBV intrauterine infection.