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目的探讨人端粒酶逆转录酶(hTERT)启动子区在肾癌患者尿沉渣中DNA甲基化状态及临床意义。方法提取76例肾癌(肾癌组)和38例非肾癌(对照组)患者术前尿沉渣中的DNA,经亚硫酸氢钠修饰后,应用甲基化特异性PCR(MSP)方法对DNA进行扩增,将MSP产物进行凝胶电泳观察分析甲基化情况。结果肾癌组患者hTERT启动子区高甲基化发生率明显高于对照组(P<0.05),但hTERT启动子区高甲基化与性别、年龄、肿瘤大小、肿瘤分期无统计学关联(P>0.05)。结论尿沉渣中hTERT启动子区甲基化与肾癌发生相关,并且其作为一种无创性检测有助于肾癌的临床诊断。
Objective To investigate the DNA methylation status of human telomerase reverse transcriptase (hTERT) promoter region in urine sediment of patients with renal cell carcinoma and its clinical significance. Methods The urine DNA from 76 patients with renal cell carcinoma (renal cell carcinoma) and 38 patients with non-renal cell carcinoma (control group) was pretreated with sodium bisulfite and methylated specific PCR (MSP) DNA amplification, the MSP product gel electrophoresis observed and analyzed methylation. Results The incidence of hypermethylation in hTERT promoter region of patients with renal cell carcinoma was significantly higher than that of the control (P <0.05), but there was no significant correlation between hypermethylation of hTERT promoter region and sex, age, tumor size and tumor stage (P> 0.05) . Conclusion The methylation of hTERT promoter in urinary sediment is related to the occurrence of renal cell carcinoma, and as a noninvasive detection, it is helpful to the clinical diagnosis of renal cell carcinoma.