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目的探讨氧化苦参碱对感染性休克大鼠心肌组织核因子-κB(NF-κB)等细胞因子的影响。方法采用大鼠盲肠结扎穿孔法制备大鼠感染性休克模型。造模后56只SD大鼠随机分为假手术组,氧化苦参碱对照组,模型组,氧化苦参碱高、中、低剂量(52、26、13 mg/kg)组、地塞米松阳性对照(10 mg/kg)组,各组给药1次。采用RT-PCR法测定心肌组织NF-κB(p65)mRNA的表达;Western blotting法测定心肌组织NF-κB(p65)及IκB-α的表达;放射免疫分析法测定心肌组织匀浆中肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)量的改变。结果氧化苦参碱能显著抑制大鼠心肌组织NF-κB(p65)mRNA的表达及NF-κB(p65)和IkB-α的活性(P<0.05),降低心肌组织匀浆中TNF-α及IL-1β的量(P<0.05)。结论氧化苦参碱能通过抑制NF-κB(p65)mRNA的表达及诱导NF-κB激酶(NF-κB-inducing kinase,NIK)的活化,抑制细菌、病毒等对NF-κB的激活作用,减少TNF-α、IL-1β等促炎因子的表达,进而对感染性休克大鼠心肌损伤性病变发挥治疗作用。
Objective To investigate the effects of oxymatrine on cytokines such as nuclear factor-κB (NF-κB) in myocardial tissue of septic shock rats. Methods Rat septic shock model was prepared by cecal ligation and puncture in rats. Fifty-six SD rats were randomly divided into sham operation group, oxymatrine control group, model group, oxymatrine high, medium and low dose (52,26,13 mg / kg) group, dexamethasone Positive control (10 mg / kg) group, each group administered 1 time. The expression of NF-κB (p65) mRNA in myocardial tissue was determined by RT-PCR. The expression of NF-κB (p65) and IκB-α in myocardium was detected by Western blotting. The levels of tumor necrosis factor -α (TNF-α) and interleukin-1β (IL-1β). Results Oxymatrine could significantly inhibit the expression of NF-κB (p65) mRNA and the activity of NF-κB (p65) and IkB-α in rat myocardium (P <0.05) The amount of IL-1β (P <0.05). Conclusion Oxymatrine can inhibit the activation of NF-κB by bacteria and viruses by inhibiting the expression of NF-κB (p65) mRNA and inducing the activation of NF-κB-inducing kinase (NIK) TNF-α, IL-1β and other proinflammatory cytokines, and then play a therapeutic role in myocardial injury in septic shock rats.