论文部分内容阅读
目的探讨CpG寡核苷酸(CpGODN)对B淋巴细胞的靶向性及免疫增强效应。方法制备CD40配体(CD40L)-多聚左旋赖氨酸(PLL)-CpGODN交联复合物。复合物与脐血单个核细胞(MNCs)共培养,流式细胞仪检测MNCs对羧基荧光素FAM标记CpGODN的摄取率、淋巴细胞亚群的表达;MTT法测MNCs增殖情况;ELISA法测培养上清IgG水平。结果与CpGODN相比,MNCs对CD40L-PLL-CpGODN交联复合物摄取率更高(>98%),达摄取峰值时间更早,细胞内荧光强度维持更稳定;MNCs与之共培养后CD19+、CD22+、CD20+细胞表达明显升高,MTT法测MNCs增殖情况、ELISA法测培养上清IgG水平均明显升高。结论CD40L-PLL载体系统能将CpGODN靶向作用于B细胞,增强其免疫效应。
Objective To investigate the targeting of CpG ODN to B lymphocytes and its immune enhancement effect. Method Preparation of CD40 Ligand (CD40L) - Poly-L-Lysine (PLL) -CpGODN Crosslinking Complex. (MNCs). The uptake of FGF labeled CpG ODN by MNCs and the expression of lymphocyte subsets were detected by flow cytometry. The proliferation of MNCs was detected by MTT assay. Clear IgG levels. Results Compared with CpG ODN, the uptake rate of MNCs on CD40L-PLL-CpGODN cross-linked complexes was higher (> 98%), the peak uptake time was earlier and the intracellular fluorescence intensity was more stable. After co-cultured with MNCs, CD19 + CD22 +, CD20 + cells were significantly increased, the proliferation of MNCs was measured by MTT assay, and the IgG level in culture supernatant was significantly increased by ELISA. Conclusion The CD40L-PLL vector system can target CpG ODN to B cells and enhance its immune effect.