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目的阐明糖尿病大鼠阴茎白膜超微结构的改变。方法实验组(DM)链脲佐菌素(STZ)诱导糖尿病的雄性SD大鼠18只,对照组(AMC)年龄相同雄性SD大鼠10只。分别于处理后5、10周测定两组大鼠血糖,并作统计学分析;5周后测量海绵体内压(ICP);两组大鼠于5、10周分别处死半数,取阴茎组织,作电镜观察。结果处理5、10周后,DM组大鼠血糖水平明显高于AMC,P<0.01。5周后, DM组ICP明显低于AMC组,P<0.01。处理周后,DM和AMC阴茎白膜厚度分别为(0.05+0.01)mm和(0.12±0.02)mm;处理10周后DM和AMC阴茎白膜厚度分别为(0.08+0.02)mm和(0.13±0.03)mm。两组分别在5周后和10周后阴茎白膜厚度比较,差异均有统计学意义P<0.05;另外可见白膜弹性纤维减少,组织紊乱,结构松驰,胶原纤维失去波形等。结论阴茎白膜超微结构改变可能损害了白膜静脉阻闭功能而导致阴茎勃起功能障碍(ED)。
Objective To elucidate the ultrastructural changes of penile white matter in diabetic rats. Methods 18 male SD rats induced by streptozotocin (STZ) in experimental group (DM) and 10 male SD rats of the same age (AMC) in control group were included in this study. The blood glucose of the two groups were measured at 5 and 10 weeks after the treatment, respectively, and statistical analysis was performed. The intracavernous pressure (ICP) was measured after 5 weeks. Half of the rats were sacrificed at 5 and 10 weeks, Electron microscopy. Results After 5 and 10 weeks of treatment, the blood glucose level in DM group was significantly higher than that in AMC group. After P <0.01.5, ICP in DM group was significantly lower than that in AMC group (P <0.01). The thickness of the penis of DM and AMC were (0.05 ± 0.01) mm and (0.12 ± 0.02) mm respectively after treatment, and the thickness of penis of DM and AMC were (0.08 ± 0.02) mm and (0.13 ± 0.03) mm. The thickness of the penile white in two groups after 5 weeks and 10 weeks were significantly different (P <0.05). In addition, there was a decrease of the white membranous elastic fibers, disorganized tissue, loose structure, and loss of the collagen fibers. Conclusion The ultrastructural changes of the penile white matter may impair the occlusion function of the white blood veins and lead to erectile dysfunction (ED).