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目的探讨加味黄芪建中汤对脾虚证肺癌小鼠血管内皮生长因子(VEGF)和微血管密度(MVD)的影响。方法多因素法建立脾虚证小鼠模型后,进行分组并接种Lewis肺癌。第10天后,取肿瘤组织,采用免疫组化法检测肿瘤组织内VEGF表达及MVD计数水平。结果脾虚证小鼠造模成功;脾虚病机使VEGF表达与MVD计数水平增加(P<0.01);加味黄芪建中汤使VEGF表达MVD计数水平降低(P<0.01)。结论加味黄芪建中汤可能通过降低VEGF的表达及MVD,进而发挥对脾虚小鼠肿瘤转移的抑制作用。
Objective To investigate the effects of Modified Astragalus Jianzhong Decoction on vascular endothelial growth factor (VEGF) and microvessel density (MVD) in mice with spleen deficiency syndrome. Methods Mice model of spleen deficiency syndrome was established by multifactorial method and then grouped and inoculated with Lewis lung cancer. After 10 days, the tumor tissues were taken and the expression of VEGF and the MVD count in tumor tissues were detected by immunohistochemistry. Results Spleen deficiency syndrome was successfully established in mice. The spleen deficiency pathogenesis increased the expression of VEGF and the level of MVD (P <0.01). Modified Astragalus Jianzhong decoction reduced the expression of VEGF in MVD (P <0.01). Conclusion Modified Astragalus Jianzhong Decoction may exert its inhibitory effect on the tumor metastasis of spleen deficiency mice by decreasing the expression of VEGF and MVD.