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目的 探讨p16 ,cyclinD1和增殖细胞核抗原 (PCNA )表达在胃癌早期诊断中的意义。方法 采用免疫组织化学 (S P)方法和显微摄像计算机图像分析技术 ,研究 185例各类胃粘膜病变和 42例早期胃癌中p16 ,cyclinD1和PCNA的表达情况。结果 p16蛋白的阳性率在慢性浅表性胃炎组 (A组 )、慢性萎缩性胃炎组 (B组 )和伴肠化生萎缩性胃炎组 (C组 )均显著高于早期胃癌组 (E组 ) (P <0 .0 5～ 0 .0 1) ;cyclinD1的阳性率A和C组均显著低于E组 (P <0 .0 5～ 0 .0 1)。结论 p16的低表达和cyclinD1的过表达在胃粘膜细胞增殖癌变过程中起重要作用 ,对胃癌的早期诊断有一定意义
Objective To investigate the significance of p16, cyclinD1 and proliferating cell nuclear antigen (PCNA) expression in the early diagnosis of gastric cancer. Methods The expression of p16, cyclinD1 and PCNA in 185 cases of gastric mucosal lesions and 42 cases of early gastric cancer were studied by immunohistochemical (S P) method and microscopic computer image analysis. Results The positive rate of p16 protein in chronic superficial gastritis group (A group), chronic atrophic gastritis group (B group) and atrophic gastritis group with intestinal metaplasia (C group) were significantly higher than that of early gastric cancer group (E group). The positive rate of cyclinD1 in group A and group C was significantly lower than that in group E (P <0.05-5.01). Conclusion The low expression of p16 and the overexpression of cyclinD1 play an important role in the proliferation and metastasis of gastric mucosal cells, which may be of significance in the early diagnosis of gastric cancer.