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在严格的厌氧条件下，用ＭＰＮ计数法测定了柴达木盆地东部第四系一个垂直剖面不同沉积层地质样品中的硫酸盐还原菌、厌氧纤维素分解菌，发酵性细菌，产甲烷细菌的数量。对各种菌的形态进行了观察，并对产甲烷细菌的代谢类型和产甲烷能力进行了研究。结果表明：柴达木东部第四系在１１３．７６－１２３．３８ｍ基本上结束了硫酸盐还原过程，业已进入了生化甲烷阶段，发酵性细菌和纤维素分解菌的分布带有很大的随机性，与样品中的有机质、纤维素的含量无明显相关关系，各样品中的菌体形态也有一定差异，产甲烷菌仅存在于部分样品中，为甲烷杆菌（Ｍｅｔｈａｎｂａｃｔｅｒｉｕｍ），营养类型为Ｈ２／ＣＯ２。
Under strict anaerobic conditions, sulfate-reducing bacteria, anaerobic cellulolytic bacteria, fermentative bacteria and methanogenic bacteria were determined by MPN counting method in the geological samples of one sedimentary layer of vertical section of Quaternary in eastern Qaidam Basin. The number of bacteria. The morphology of various bacteria was observed, and the methanogens metabolism and methanogenic ability were studied. The results show that the Quaternary in the east of Qaidam basically ended the sulfate reduction process at 113.76-123.38 m, entered the stage of biochemical methane, and the distribution of fermentative bacteria and cellulolytic bacteria was very random There is no significant correlation between the morphology and the content of organic matter and cellulose in the sample. The morphology of the bacteria in each sample is also different. The methanogen is only existed in some samples, which is Methanbacterium, the nutrient type is H2 / CO2.