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目的探讨长期吸烟对大鼠的肺损伤及相关炎性因子表达的影响。方法 56只雄性SD大鼠,随机分为对照组和低、中、高3个剂量吸烟组,采用自主开发的吸烟机给烟12周;放免法检测支气管肺灌洗液(BALF)中白介素-8(IL-8)的含量,免疫组化法检测肺血管内皮细胞E-选择素的表达水平。结果高、中、低剂量吸烟组大鼠BALF中IL-8的含量分别为(0.77±0.010)、(0.71±0.171)、(0.62±0.088)ng/mL,高于对照组(0.28±0.133))ng/mL,差异有统计学意义(P<0.01);高、中、低剂量吸烟组E-选择素的表达分别为(0.27±0.030)、(0.18±0.034)、(0.16±0.025),均高于对照组(0.07±0.023),差异有统计学意义(P<0.01);中剂量吸烟组肺血管内皮细胞E-选择素与BALF中IL-8的含量呈明显正相关(r=0.716,P<0.01)。结论长期吸烟导致气道炎症介质IL-8和E-选择素的表达升高,促进气道内炎症反应,对支气管肺组织造成严重损害。
Objective To investigate the effects of long-term smoking on lung injury and the expression of related inflammatory factors in rats. Methods Fifty-six male Sprague-Dawley rats were randomly divided into three groups: control group, low, medium and high dose of three groups, smoking for 12 weeks with self-developed smoking machine. The levels of interleukin- 8 (IL-8) were detected by immunohistochemistry. The expression of E-selectin in pulmonary vascular endothelial cells was detected by immunohistochemistry. Results The levels of IL-8 in BALF were (0.77 ± 0.010), (0.71 ± 0.171) and (0.62 ± 0.088) ng / mL, respectively, higher than those in control group (0.28 ± 0.133) (P <0.01). The expressions of E-selectin in high, medium and low dose groups were (0.27 ± 0.030), (0.18 ± 0.034) and (0.16 ± 0.025) (P <0.01). There was a significant positive correlation between E-selectin and IL-8 in BALF in middle-dose group (r = 0.716 , P <0.01). Conclusion Long-term smoking leads to elevated expression of interleukin-8 and E-selectin in airway inflammatory mediators, promotes airway inflammation and causes severe damage to bronchopulmonary tissues.