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目的探讨脆性组氨酸三联体(FHIT)基因和PTEN基因在原发性肝细胞癌(简称肝癌)中的表达及其与临床病理因素的关系。方法采用免疫组织化学SP法和图像分析技术检测了46例肝癌组织及癌旁组织和10例正常肝组织中FHIT和PTEN基因蛋白的表达。结果46例肝癌组织中FHIT和PTEN蛋白免疫组化阳性反应颗粒的平均光密度分别为(0.1339±0.3864)、(0.1534±0.1562),均明显低于相应癌旁组织(0.2051±0.5372)、(0.3271±0.2371)和正常组织(0.2289±0.7589)、(0.3753±0.0519)的水平(P<0.05);FHIT、PTEN与肝癌的病理分级、合并门静脉癌栓有密切关系。结论FHIT和PTEN基因蛋白缺失在肝癌组织中是频发事件。FHIT和PTEN与肝癌的病理分级、合并门静脉癌栓有密切关系。
Objective To investigate the expression of FHIT gene and PTEN gene in primary hepatocellular carcinoma (HCC) and its relationship with clinicopathological factors. Methods The expression of FHIT and PTEN protein in 46 cases of HCC tissues and adjacent normal tissues and 10 cases of normal liver tissues were detected by immunohistochemical SP method and image analysis technique. Results The average optical density of FHIT and PTEN positive immunohistochemical particles in 46 HCC tissues were (0.1339 ± 0.3864) and (0.1534 ± 0.1562), respectively, which were significantly lower than those in the corresponding paracancerous tissues (0.2051 ± 0.5372, 0.3271 ± 0.2371) and normal tissues (0.2289 ± 0.7589) and (0.3753 ± 0.0519), respectively (P <0.05). FHIT and PTEN were closely related to the pathological grade of hepatocellular carcinoma and portal vein tumor thrombus. Conclusion The deletion of FHIT and PTEN gene is a frequent event in HCC tissues. FHIT and PTEN with the pathological grade of liver cancer, with portal vein tumor emboli are closely related.