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目的通过体外实验比较不同产地的甘草提取液对乙型肝炎病毒(HBV)的抑制作用,为甘草药材的质量评价及抗病毒新药的研发提供科学依据。方法体外培养人肝癌细胞株(Hep G2.2.15)细胞,以拉米夫定作阳性对照药物,噻唑蓝(MTT)法检测甘肃、内蒙古甘草、甘草酸、甘草苷对细胞的生长抑制作用,分别计算抑制率和半数毒性浓度(TC50);酶联免疫吸附(ELISA)法检测药物对细胞表达的乙型肝炎表面抗原(HBs Ag)和乙型肝炎e抗原(HBe Ag)的影响,分别计算抑制率和半数抑制浓度(EC50),最后计算治疗指数(TI)以进行抗HBV作用评价。结果甘肃、内蒙古甘草具有抑制Hep G2.2.15细胞生长,随着药物浓度增大,其抑制率也随之增大。甘肃甘草对HBs Ag、HBe Ag抑制作用的TI值为11.570、6.760,内蒙古甘草为8.360、5.020。结论甘肃、内蒙古甘草提取液在体外均有明显抑制HBV作用,甘肃甘草作用更强。
OBJECTIVE: To compare the inhibitory effects of licorice extract from different habitats on Hepatitis B virus (HBV) in vitro and to provide a scientific basis for the quality evaluation of licorice herb and the development of new antiviral drugs. Methods Human hepatocellular carcinoma cell line Hep G2.2.15 was cultured in vitro. Lamivudine was used as a positive control drug and the growth inhibition of Glycyrrhiza glabra glycyrrhizinate and glycyrrhizin were detected by MTT assay. The inhibition rate and half-value toxic concentration (TC50) were calculated. The effect of drugs on HBsAg and HBeAg expression was detected by enzyme-linked immunosorbent assay (ELISA) Rate and half inhibitory concentration (EC50), and finally calculate the therapeutic index (TI) for anti-HBV evaluation. Results Gansu and Inner Mongolia licorice could inhibit the growth of Hep G2.2.15 cells. With the increase of drug concentration, the inhibition rate also increased. The inhibitory effects of Glycyrrhiza glabra on HBsAg and HBeAg were 11.570,6.760 and that of Inner Mongolia licorice was 8.360,5.020. Conclusion Gansu, Inner Mongolia licorice extract in vitro significantly inhibited the role of HBV, licorice Gansu stronger role.