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稀释结晶法是Ｒｕｂｉｓｃｏ结晶的有效方法。稀释结晶与Ｒｕｂｉｓｃｏ的沉淀提取步骤中的离子强度关系密切。ｐＨ５．４沉淀Ｒｕｂｉｓｃｏ，提高沉淀过程中的离子强度，有利于提高Ｒｕｂｉｓｃｏ沉淀的复溶率与结晶率和提高Ｒｕｂｉｓｃｏ结晶时ｐＨ与离子强度选择范围，使Ｒｕｂｉｓｃｏ的稀释结晶提取方法更有效便捷。ｐＨ５．０下沉淀Ｒｕｂｉｓｃｏ，其复溶稀释结晶耗时长、收率低、方法不稳定。通过提高沉淀过程中的离子强度，其复溶液中Ｒｕｂｉｓｃｏ的复溶率与结晶率均较对照有显著提高。Ｒｕｂｉｓｃｏ沉淀过程中的离子强度直接影响到Ｒｕｂｉｓｃｏ沉淀物的规则性、沉淀的复溶率、复溶Ｒｕｂｉｓｃｏ的Ａ２８０／Ａ２６０值、ＯＤ３９０值，对结晶速率、结晶率以及结晶对ｐＨ与离子强度条件的选择范围均有重要影响。离子强度的选择控制在０．６ｍｏｌ·Ｌ－１－１．５ｍｏｌ·Ｌ－１ＮａＣｌ合适，过低与过高均不利于Ｒｕｂｉｓｃｏ的结晶。
Dilution crystallization is an effective method for the crystallization of Rubisco. The dilution crystallization is closely related to the ionic strength in the precipitation step of Rubisco. The precipitation of Rubisco at pH5.4 improves the ionic strength during the precipitation process, which helps to improve the recovery rate and crystallization rate of Rubisco precipitation and the range of pH and ionic strength when Rubisco is crystallized, which makes the extraction method of Rubisco diluted and crystallized more effective and convenient. Under the condition of pH5.0, Rubisco is precipitated, the dilution and crystallization of the reconstituted solution takes a long time and the yield is low, and the method is unstable. By increasing the ionic strength in the precipitation process, the recovery rate and the crystallization rate of Rubisco in the complex solution were significantly increased compared with the control. The ionic strength of Rubisco precipitation process has a direct impact on the regularity of Rubisco sediment, the re-precipitation rate, A280 / A260 value and OD390 value of Rubisco, and the crystallization rate, crystallization rate and crystallization to pH and ionic strength The range of choices has important implications. The selection of ionic strength is controlled at 0.6mol · L-1-1.5mol · L-1NaCl appropriate, too low and too high are not conducive to the crystallization of Rubisco.