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目的 :研究六亚甲基双乙酰胺 (HMBA)对涎腺粘液表皮样癌高转移细胞 Mc3恶性表型的影响。方法 :采用 MTT法、细胞计数法、克隆形成法、流式细胞术及癌细胞裸鼠尾静脉注射法 (每只鼠 10 6个细胞 )研究HMBA对 Mc3细胞生长以及转移力的抑制作用。结果 :HMBA在 1mmol/ L 时对 Mc3细胞生长抑制率为36 % ,作用于 Mc3细胞 5 d后对照组和处理组细胞倍增时间 (h)分别为 2 0 .8和 2 3.5 ,S细胞比例 (% )为 2 4.5和2 3.0。野生型 P5 3蛋白表达率 (% )为 2 4.0和 99.7,nm2 3- H1 蛋白表达率 (% )为 99.9和 98.9,克隆形成率 (% )为 2 3.5和 16 .0 ,在裸鼠肺表面转移结节数为 139± 6 1和 83± 37。结论 :HMBA可抑制 Mc3细胞的转移力 ,其机制可能与诱导分化作用有关
Objective: To study the effect of hexamethylene bisacetamide (HMBA) on the malignant phenotype of high metastatic cells in salivary mucoepidermoid carcinoma. METHODS: MTT assay, cell counting, cloning method, flow cytometry, and tail vein injection of cancer cells (106 cells per mouse) were used to study the inhibitory effect of HMBA on the growth and migration of Mc3 cells. Results: The growth inhibition rate of Mc3 cells at 1mmol/L of HMBA was 36%. The cell doubling time (h) of control cells and treatment groups after 2 days of Mc3 cells was 20.8 and 23.5, respectively. %) is 2 4.5 and 2 3.0. The wild-type P5 3 protein expression rates (%) were 2 4.0 and 99.7, the nm2 3-H1 protein expression rate (%) was 99.9 and 98.9, and the colony formation rate (%) was 2 3.5 and 16.0, on the lung surface of nude mice. The number of metastatic nodules was 139 ± 61 and 83 ± 37. Conclusion: HMBA can inhibit the migration of Mc3 cells. The mechanism may be related to the induction of differentiation.