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为探讨CD40L在儿童川崎病发病机制中的作用,用流式细胞技术及ELISA法检测急性期川崎病(KD)患儿外周血单个核细胞(PBMC)体外表达CD40L水平、细胞凋亡率及可溶性CD40L(sCD40L)对单核细胞株THP-1产生TNF-α的影响。结果:KD表达CD40L明显增高;存在淋巴细胞凋亡延迟;患儿PBMC培养上清(可能含有sCD40L)可诱导THP-1产生高浓度的TNF-α。抗CD40L单抗可纠正上述异常效应。CD40L的异常表达在川崎病的发病中起重要作用,抗CD40L单抗对KD 具有潜在的免疫学治疗前景。
To investigate the role of CD40L in the pathogenesis of Kawasaki disease in children, flow cytometry and ELISA were used to detect the expression of CD40L in peripheral blood mononuclear cells (PBMCs) in children with acute Kawasaki disease (KD) in vitro. The apoptosis rate and soluble Effect of CD40L (sCD40L) on the production of TNF-α by monocyte strain THP-1. Results: KD expression of CD40L was significantly increased; there was delayed apoptosis of lymphocytes; PBMC culture supernatant of children (possibly containing sCD40L) could induce THP-1 to produce high concentration of TNF-α. Anti-CD40L mAb can correct the above abnormal effects. Aberrant expression of CD40L plays an important role in the pathogenesis of Kawasaki disease. Anti-CD40L mAb has potential immunological prospect for KD.