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[目的]探析谷子调控干旱胁迫的E3基因。[方法]本文基于二代测序技术获得勾勾母鸡咀(GG)和晋汾16(JF)在PEG胁迫下的转录表达谱数据,运用生物信息学检索筛选出11个干旱相关的E3基因,对其理化性质、功能域、启动子元件、表达模式以及与其它物种E3基因的系统进化分析。[结果]蛋白等电点介于4.78~8.88之间,功能域主要为ZF_UBR型、ZF_RING和U_BOX型三类,启动子序列包含干旱诱导响应元件MBS或脱落酸响应元件ABRE等,其中Si001665m和Si022170m与PUB22/23亲缘关系较近,Si029090m与PUB19亲缘关系较近。[结论]初步确定这些是调控谷子干旱胁迫的E3候选基因,可能受干旱或脱落酸诱导表达,这为进一步研究谷子干旱响应相关的泛素连接酶E3基因提供理论依据。
[Objective] The research aimed to analyze the E3 gene of millet regulating drought stress. [Method] The transcriptional profiling data of GG and JF under PEG stress were obtained based on the second-generation sequencing technology. Eleven drought-related E3 genes were screened by bioinformatics methods. Phylogenetic analysis of its physicochemical properties, functional domains, promoter elements, expression patterns, and E3 genes with other species. [Result] The isoelectric point of protein ranged from 4.78 to 8.88. The main functional domains were ZF_UBR, ZF_RING and U_BOX. The promoter sequence contained the drought-responsive response element MBS or abscisic acid responsive element ABRE. Among them, Si001665m and Si022170m The genetic relationship with PUB22 / 23 is relatively close, Si029090m has a close relationship with PUB19. [Conclusion] It is preliminarily determined that these are E3 candidate genes regulating the drought stress of millet, which may be induced by drought or abscisic acid. This provides a theoretical basis for further studying the ubiquitin ligase E3 gene related to drought response of millet.