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内毒素自鼠伤寒杆菌中成功的被提取出以来,众多学者对内毒素的理化特性,生物学效应和其在疾病过程中所起的作用有了一定的认识。近年来,随着抗生素的广泛使用,G~+细菌感染性疾病已逐渐减少,而G~-细菌感染性疾病则相应增多,这种感染大多发病急,病情重,常致内毒素休克。一旦内毒素休克发生,死亡率常常很高。本实验对几种常用的清热解毒中草药,进行了体外抗内毒素效力的比较,力图筛选出高效抗内毒素之药物。材料与方法一、中药:由天津市药材公司购进中药双花、连翘、公英、地丁、败酱草。取各单味药的提取液,经灭菌离心后取上清液备用。二、实验方法:内毒素检测采用基质显色,法。所用药物及配套试剂由上海医化所和上海生物制品研究所提供。实验方法取上述各中药药液0.1ml,加入已知量内毒素溶液0.1ml,37℃温育。
Since endotoxin was successfully extracted from S. typhimurium, many scholars have a certain understanding of the physical and chemical properties of endotoxin, biological effects and its role in the disease process. In recent years, with the widespread use of antibiotics, G~+ bacterial infectious diseases have gradually decreased, and G~-bacterial infectious diseases have increased correspondingly. Most of these infections are acute and severe, often causing endotoxin shock. Once endotoxin shock occurs, the mortality rate is often high. In this experiment, several commonly used heat-clearing and detoxifying Chinese herbal medicines were compared for the efficacy of in vitro anti-endotoxins in an attempt to screen for highly potent anti-endotoxin drugs. Materials and Methods First, Chinese medicine: Tianjin Traditional Chinese Medicine Company purchased traditional Chinese medicines such as Double Flower, Forsythia, Gongying, Didi, and Patrinia. Take the extracts of each single herb, sterilize and centrifuge, then take the supernatant for use. Second, experimental methods: Endotoxin detection using matrix color, method. The drugs and supporting reagents used were provided by Shanghai Institute of Medical Chemistry and Shanghai Institute of Biological Products. Experimental methods Take 0.1ml of each of the above Chinese herbal medicines, add 0.1ml of a known amount of endotoxin solution, and incubate at 37°C.