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目的利用人结肠癌标本检测磷酸神经鞘氨醇(sphingosine 1-phosphate,s1p)及其相关激酶sphk1、sphk2的表达,研究其作为血清标记物判断结肠癌预后肿瘤标志物的价值。方法利用real-time PCR检测s1p及其相关激酶sphk1、sphk2的表达,免疫组织化学染色检测蛋白的表达及定位,并利用线性相关性分析及Kaplan-Meier曲线分析结肠癌患者血清s1p与预后的关系。结果 s1p及其受体相关激酶在人结肠癌中表达增高;sphk1在结肠癌组织中高表达;sphk2在正常组织中高表达,在结肠癌组织中表达下降;s1p的表达与sphk1呈正相关,而与sphk2的表达呈负相关;血清s1p的表达与患者的预后呈负相关。结论 s1p在结肠癌中高表达,是判断结肠癌患者术后差的生物标志物。
Objective To detect the expression of sphingosine 1-phosphate (s1p) and its related kinases sphk1 and sphk2 in human colon cancer specimens and to evaluate the value of sphk1 and sphk2 as tumor markers in predicting colon cancer prognosis. Methods The expression of s1p, sphk1 and sphk2 were detected by real-time PCR. The expression and localization of sphk1 and sphk2 were detected by immunohistochemical staining. The relationship between s1p and the prognosis of colon cancer patients was analyzed by linear correlation analysis and Kaplan-Meier curve . Results The expression of s1p and its receptor-associated kinases in human colon cancer increased, sphk1 was highly expressed in colon cancer tissues, sphk2 was overexpressed in normal tissues and decreased in colon cancer tissues. The expression of s1p was positively correlated with sphk1 but not with sphk2 The expression of s1p was negatively correlated with the prognosis of patients. Conclusion s1p is highly expressed in colon cancer and is a biomarker for postoperative poor judgment of colon cancer patients.