目的:通过ABO血型基因分析,协助临床血清学疑难血型标本的鉴定。方法:血型血清学方法采用凝胶卡法,疑难的标本采用试管法进一步分析鉴定;同时进行血型基因测序分析。结果:15例疑难血型标本中出现CisAB01、B(A)02、ABmh类孟买型等位基因标本。结论:采用基因分析的方法,可以提供疑难血型血清学标本表型鉴定的佐证,确认血清学不易检测的结果,更好的保证输血安全。 OBJECTIVE: To assist in the identification of clinically difficult blood group specimens by ABO blood group gene analysis. Methods: Serum card method was used for blood group serology, and the difficult specimens were further analyzed and identified by in vitro test. Meanwhile, blood group gene sequencing analysis was performed. Results: CisAB01, B (A) 02 and ABmh Mumbai type alleles were found in 15 cases of difficult blood type. Conclusion: The method of gene analysis can provide evidence of phenotypes of difficult blood group serological specimens, confirm the results of not easy to detect serology, and better ensure the safety of blood transfusion.