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目的建立以维吾尔医学基础理论为指导的异常黑胆质型绝经后骨质疏松大鼠模型,评价该动物模型的有效性。方法选取雌性Wistar大鼠48只,随机分为正常对照组、去势组、异常黑胆质型组、异常黑胆质型绝经后骨质疏松组,除正常对照组外,其余各组大鼠用干寒属性饲养环境和干寒属性饲料饲养,以及间断性足底电击等多因素共同作用,建立异常黑胆质证载体大鼠模型,以此为基础采用卵巢切除法建立异常黑胆质型绝经后骨质疏松大鼠模型,通过异常黑胆质证载体动物模型评价方法,检测各组大鼠第二腰椎及胫骨上端的骨密度,对此造模方法的可行性和可重复性进行综合评价。结果与正常对照组比较,异常黑胆质型绝经后骨质疏松组具有异常黑胆质证候特征的一般状态,符合多因素共同作用建立的异常黑胆质载体动物模型,且第二腰椎和胫骨上端骨密度明显降低(P<0.05),出现绝经后骨质疏松的变化。结论本研究成功建立异常黑胆质型绝经后骨质疏松大鼠模型,并具有稳定性、可靠性、可重复性。
Objective To establish an animal model of abnormal savda-type osteoporosis induced by basic medical theory of Uyghur to evaluate the effectiveness of the animal model. Methods Forty-eight female Wistar rats were randomly divided into normal control group, castration group, abnormal savda type group and abnormal savda type postmenopausal osteoporosis group. Except normal control group, rats in other groups Using dry and cold feeding environment and dry and cold feed breeding, and intermittent foot shock and other factors to establish an abnormal Savda vector rat model, based on which ovariectomy to establish abnormal savda-type menopause After osteoporosis rat model, the animal model of abnormal savda will be used to evaluate the bone mineral density of the second lumbar vertebrae and the upper tibia of rats in each group. The feasibility and repeatability of this model are evaluated. Results Compared with the normal control group, the group of abnormal savda type postmenopausal osteoporosis had abnormal savda syndrome characteristics, which accorded with the animal model of abnormal savda carrier established by multiple factors and the second lumbar and tibia The density of upper bones decreased significantly (P <0.05), and the change of postmenopausal osteoporosis appeared. Conclusion This study successfully established an abnormal Savda model of postmenopausal osteoporosis rats, and has the stability, reliability and repeatability.