了解游离氨基酸代谢储备量的大小和它的组成,对解决在不同因素影响下,人对蛋白质需要的问题是很重要的。作者用滤纸层析法测定了健康人血液中的游离氨基酸的含量,以便迸而研究在不同的条件下各个氨基酸的含量可能发生的变化。 作者对47名年令20—27岁的健康男子进行了研究。受试者摄取同样的膳食一昼夜膳食中含蛋白质约140g。早晨空腹时自臂静脉取血制成血清,取2ml血清,加入乙醇以沉淀蛋白 Understanding the size of the free amino acid metabolism reserve and its composition is important to solve the problem of human protein needs under the influence of different factors. The authors used filter paper chromatography to determine the content of free amino acids in healthy human blood in order to investigate the possible changes in the content of individual amino acids under different conditions. The authors studied 47 men aged 20-27 years. The subjects consumed the same diet for about 140 g of protein in a day and night meal. Serum was taken from the brachiocephalic vein in the morning on an empty stomach, serum was taken 2ml, ethanol was added to precipitate the protein